<p>(A) Fluorescence microscopy imaging of ZipA-Gfp rings in cells expressing ZipA-Gfp from the chromosome induced with 10 μM IPTG in strains with (MC181) and without <i>clpX</i> (MV0226) under growth conditions described in <i>Materials and methods</i>. Size bar is 2 μm. (B) Box and whiskers plot of average recovery half-times of ZipA-Gfp rings with (MC181) and without <i>clpX</i> (MV0226). The extent of the box encompasses the interquartile range of the fluorescence recovery half-times, and whiskers extend to the maximum and minimum values. The line within each box represents the median. Where indicated, <i>p</i> values are specified as ‘n.s.’ (not significant) as compared to wild type.</p
<p>(<b>A</b>) Digital fluorescent micrographs of the nucleus of a cell expressing GFP-H3.3 before an...
<p><b>A</b> Average Sla1-GFP patch lifetimes in the wild-type, and cells expressing AtxA, AtnL, and ...
<p>(A) Diagram of experiment. Fluorescence in daughter cells was photobleached and the time course o...
<p>(A) A region of the Z-ring was selected and bleached from wild type cells (JC0390) expressing Gfp...
<p>Cartoon in (A) outlines the induction experiment designed to explore the dynamics of the full-len...
<p>(A) Experimental set up. SCs are followed by Zip1-GFP; SPBs by Cnm67-mCherry, and anaphase I onse...
<p>(A) Cells harboring <i>ftsA<sub>R286W</sub></i> to allow bypass of <i>zipA</i> (Δ<i>zipA::aph</i>...
<p>Cartoon in (A) outlines the induction experiment designed to ask whether Zip1 subunits exit the f...
<p>(A) Cartoon outlines the induction experiment carried out using K29 and K30. Meiotic cells expres...
<p>(A) The <i>zipA<sub>L286Q</sub></i> allele is a spontaneously isolated mutant resistant to loss o...
<p>(A) A composite of ZipA-GFP (green), DAPI stained nucleoid (red) and phase contrast images (grey)...
<p>(A) In the first row, fluorescence (left) and overlay between fluorescence and phase contrast ima...
MEFs from Clta-eGFP mice show dynamic puncta consistent with Clta-eGFP localization at coated pits a...
<p>(A) Zip1 localization is discontinuous on meiotic prophase chromosomes in the <i>ecm11</i> and <i...
<p>A) MDA-MB-231 cells transfected with GFP-actin, double stained with Phalloidin-Alexa 594 (a). Act...
<p>(<b>A</b>) Digital fluorescent micrographs of the nucleus of a cell expressing GFP-H3.3 before an...
<p><b>A</b> Average Sla1-GFP patch lifetimes in the wild-type, and cells expressing AtxA, AtnL, and ...
<p>(A) Diagram of experiment. Fluorescence in daughter cells was photobleached and the time course o...
<p>(A) A region of the Z-ring was selected and bleached from wild type cells (JC0390) expressing Gfp...
<p>Cartoon in (A) outlines the induction experiment designed to explore the dynamics of the full-len...
<p>(A) Experimental set up. SCs are followed by Zip1-GFP; SPBs by Cnm67-mCherry, and anaphase I onse...
<p>(A) Cells harboring <i>ftsA<sub>R286W</sub></i> to allow bypass of <i>zipA</i> (Δ<i>zipA::aph</i>...
<p>Cartoon in (A) outlines the induction experiment designed to ask whether Zip1 subunits exit the f...
<p>(A) Cartoon outlines the induction experiment carried out using K29 and K30. Meiotic cells expres...
<p>(A) The <i>zipA<sub>L286Q</sub></i> allele is a spontaneously isolated mutant resistant to loss o...
<p>(A) A composite of ZipA-GFP (green), DAPI stained nucleoid (red) and phase contrast images (grey)...
<p>(A) In the first row, fluorescence (left) and overlay between fluorescence and phase contrast ima...
MEFs from Clta-eGFP mice show dynamic puncta consistent with Clta-eGFP localization at coated pits a...
<p>(A) Zip1 localization is discontinuous on meiotic prophase chromosomes in the <i>ecm11</i> and <i...
<p>A) MDA-MB-231 cells transfected with GFP-actin, double stained with Phalloidin-Alexa 594 (a). Act...
<p>(<b>A</b>) Digital fluorescent micrographs of the nucleus of a cell expressing GFP-H3.3 before an...
<p><b>A</b> Average Sla1-GFP patch lifetimes in the wild-type, and cells expressing AtxA, AtnL, and ...
<p>(A) Diagram of experiment. Fluorescence in daughter cells was photobleached and the time course o...