Localization of ZipA-GFP labeled Z-rings relative to cell center and the center of nucleoids.

<p>(A) A composite of ZipA-GFP (green), DAPI stained nucleoid (red) and phase contrast images (grey) of a Δ<i>slmA</i> Δ<i>min</i> cell with a distinctly off-center placed nucleoid. The scale bar is 2 µm. (B) The intensity line profiles of each image plane along the long axis of the cell for the cell shown in panel A. The displacement of the nucleoid relative to the cell center is ΔX_n, and the displacement of the ZipA-GFP labeled Z-ring is ΔX_z. (C) ΔX_z vs. ΔX_n for Δ<i>slmA</i> Δ<i>min</i> cells (strain TB86) scaled by cell length L. Solid rectangles mark central and open rectangles mark polar Z-rings. The solid line corresponds to . Data are shown only for cells with a single nucleoid. (D) ΔX_z vs. ΔX_n for the parental strain (strain JMBW5). (E), (F) Distribution of distances between the Z-ring center and nucleoid center for Δ<i>slmA</i> Δ<i>min</i> strain and parental strain, respectively. Data for central Z-rings are shown. (G), (H) ΔX_z vs. ΔX_n for cells that show a Z-ring over a compact nucleoid in Δ<i>slmA</i> Δ<i>min</i> and in parental strain, respectively.