Overexpressed Pin4C reduces the transmission of Sup35-GFP from mother to daughter cells.

<p>(A) Diagram of experiment. Fluorescence in daughter cells was photobleached and the time course of fluorescence recovery of the daughter cells was measured as described previously <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003236#pgen.1003236-KawaiNoma1" target="_blank">[70]</a>. (B–D) Quantitative FRAP analysis of Sup35-GFP in 10 [<i>psi</i>⁻][<i>pin</i>⁻] (GF658) cells (B), 9 strong [<i>PSI</i>⁺][<i>PIN</i>⁺] (GF657) cells harboring the pHR81GAL vector (C), and 12 strong [<i>PSI</i>⁺][<i>PIN</i>⁺] (GF657) cells harboring pHR81GAL-<i>PIN4C</i> and containing enlarged Sup35-GFP foci following overnight Pin4C overexpression (D). The relative fluorescence intensity (RFI) of the bleached daughter cell was determined every 5 s after completion of photobleaching and normalization. Error bars indicate the standard error of the mean. RFI in (D) represents the average of 3 cells (I), 4 cells (II) and 3 cells (III). The analysis of two more cells is not shown. (E) The recovery plateau level and half-time from the curves in B–D are listed.