Zip1 disappearance is delayed in <i>ipl1-mn</i> mutants.

<p>(A) Experimental set up. SCs are followed by Zip1-GFP; SPBs by Cnm67-mCherry, and anaphase I onset by Pds1-tdTomato (confluent staining). (B) Representative montages of SC disassembly (loss of Zip1-GFP), SPB separation (CNM67-mCherry), and anaphase I onset (degradation of Pds1-tdTomato) in wild type (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0083982#pone.0083982.s002" target="_blank">Movie S1</a>) and <i>ipl1-mn</i> (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0083982#pone.0083982.s003" target="_blank">Movie S2</a>). Bars: 2 µm. (C) The proportion of cells with linear Zip1-GFP structures, diffuse Zip1-GFP staining or no Zip1-GFP signal at anaphase I onset (Pds1 degradation). (D) Time from SC disassembly (Zip1-GFP signal loss) to separation of the SPBs. (E) Time from SC disassembly to anaphase I onset (Pds1 degradation). Strains: WT, Y4044 and <i>ipl1-mn</i>, Y4047.