Additional file 1: Table S1. of Grandparental immune priming in the pipefish Syngnathus typhle

Additional values of 2-way PERMANOVA output. Multivariate PERMANOVA analysis to assess the effect and interaction of two fixed factors F0-sex and F2-bacteria while including size as covariate and family as strata term on relative gene expression data (−∆Ct-values). Each analysis was based on an Euclidean distance matrix with p-values obtained by 10000 permutations. Significant p-values are marked in bold letters and asterix symbol (significance code: <0.001***, 0.001**, 0.01*, 0.1 > p-value ≥ 0.05 trend ●). R2 value indicate the percentage of variance explained by the model. Table S2. Results from PERMANOVA and ANOSIM analysis of one-week-old F2-juveniles per functional gene categories. Multivariate ANOSIM was performed following significant PERMANOVA effects to assess differences in the gene expression profiles per treatment groups applying pairwise comparison on relative gene expression data (−∆Ct-values) based on a Euclidean distance matrix and 10000 permutations. Pairwise comparison was conducted for following fixed factors and their interactions: F0-sex (grandparental (F0-Bi), grand-maternal (F0-Mat), grand-paternal (F0-Pat), grandparental control (F0-N)) and F2-bacteria (F2-bacteria control (F2-N), F2-bacteria Vibrio (F2-V+) and F2-bacteria Tenacibaculum (F2-T+)).(DOCX 56 kb