Additional file 1: of Acting locally - affecting globally: RNA sequencing of gilthead sea bream with a mild Sparicotyle chrysophrii infection reveals effects on apoptosis, immune and hypoxia related genes

A) PCR-array results: Mean and SEM of the fold changes of the 45 selected genes in Sparicotyle infected fish (n = 10) calculated relative to the control uninfected group (n = 10) in parasitized gill portions (with parasite presence), non-parasitized gill portions (no parasite present) and spleen. Student’s t test or Mann-Whitney-Wilcoxon test when normality conditions were not met were used to calculate p values comparing expression levels of control and infected animals per gene. Dark red and green indicate significantly up- or down-regulated genes, respectively, with p < 0.05. Light red and green indicate up- or down-regulated genes, respectively, with p < 0.1. The first column (Pathway) indicates the main pathway with which each gene is related. These pathways were targeted due to the nature of the specific disease studied. B) Information on the primers used in this study. (PDF 708 kb