Characterization of adipocyte-specific <i>Arrdc3</i>-null mice.

<p>(A) To confirm adipocyte-specific deletion, Arrdc3 expression was measured in various tissues of Cre–(control) and Cre+ (<i>Arrdc3</i>-null) mice by quantitative PCR. Brown (BAT), parametrial (VAT) and subcutaneous adipose tissue (SAT) had significantly decreased <i>Arrdc3</i> expression while there was no significant difference in liver or kidney (n = 3–4). (B) Adipocyte-specific <i>Arrdc3</i>-null mice and littermate controls were weighed for 16 weeks and no differences in body weight were found (n = 4–10). (C) Specific adipose depots of female mice were weighed and normalized to total body weight. Subcutaneous (SAT) and parametrial (VAT) adipose tissue from adipocyte-specific <i>Arrdc3</i>-null mice weighed significantly less than controls (n = 5). (D) Representative macroscopic (formaldehyde fixed tissue) and microscopic appearance of subcutaneous (SAT), parametrial (VAT) and brown (BAT) adipose tissue from adipocyte-specific <i>Arrdc3</i>-null and control mice. Paraffin tissue sections were stained with hematoxylin and eosin and images were taken at 40x.