Immune cell infiltration of adipose tissue during obesity is not altered in AdpnCre⁺ Rae⁺ mice.

<p>(A) The epididymal adipose tissue from AdpnCre⁺ Rae⁺ and control mice were analyzed by quantitative PCR to determine Rae-1 expression. The results were normalized to β-actin levels and are shown as fold-increase over AdpnCre⁻ Rae⁻ mice. (B) Splenocytes, peripheral blood mononuclear cells (PBMC), and stromal vascular fractions isolated from the epididymal adipose tissue of AdpnCre⁻ Rae⁺ and AdpnCre⁺ Rae⁺ mice were stained with NKG2D antibodies and analyzed by flow cytometry. Cells were gated on 7AAD⁻, CD45⁺, CD3ε⁻, NK1.1⁺ populations. (C) The stromal vascular fraction isolated from the epididymal adipose tissue of AdpnCre⁺ Rae⁻ and AdpnCre⁺ Rae⁺ mice after 5 weeks on the diet regimen were stained with antibodies and analyzed by flow cytometry. The cells were gated on 7AAD⁻, CD45⁺ populations. The data represent mean ± SD of 3 independent experiments.