Concentration and time-dependent responses of microglia in IL1β release upon LPS plus BzATP stimulation. a IL1β secretion was detected in LPS-primed microglia with different concentrations of BzATP for 2 h. b IL1β secretion was detected in LPS-primed microglia with 380 μM BzATP for different time points. One-way ANOVA followed by Tukey’s post hoc test. ***P < 0.001; ****P < 0.0001. (DOCX 121 kb
Figure S1. Purity of microglia cells in culture assessed by immunofluorescence detection of Iba-1- (...
Figure S2. Efficient and selective inactivation of MFP2 in microglia in Cx3cr1-Mfp2−/− mice. (A) Mic...
Figure S1. Treatment of rat cortical microglia with Apo-SAA or LPS upregulates, in a time-dependent ...
Figure S3. P2X7Rs do not regulate microglial intracellular pH, IL-1β/IL-18 release, blebbing, or pho...
Figure S2. Microglia P2X7Rs are dynamically regulated by bacterial phagocytosis. (a) The current-vol...
Supplementary figures for cytokines in conditioned medium from microglia show significant upregulati...
Figure S4. P2X7R dye uptake is independent of cell lysis and selective for cations below a certain t...
Figure S2. Simultaneous stimulation of primary microglia with LPS plus IL4. * p < 0.05, ** p < 0.01,...
Cultured human microglia do not bleb in response to ATP. Microglia cultured on chamber slides were e...
PKD family inhibition abrogates LPA-mediated downstream signaling. (A) BV-2 microglia cells were cul...
Figure S1. Survival of primary microglia in the presence of inflammatory mediators. * p < 0.05, ** p...
LPAR5 controls LPA-mediated downstream signaling. (A) BV-2 microglia cells were cultured in 6-well p...
Figure S2. Immunoblotting data of main Fig. 2c, d and f were quantified using Image J software and s...
Figure S1. Immunoblotting data of main Fig. 1a-c were quantified using Image J software. Data were t...
Effects of the AM630 on PGE2 release in LPS-stimulated primary microglial cells. Microglia were pre-...
Figure S1. Purity of microglia cells in culture assessed by immunofluorescence detection of Iba-1- (...
Figure S2. Efficient and selective inactivation of MFP2 in microglia in Cx3cr1-Mfp2−/− mice. (A) Mic...
Figure S1. Treatment of rat cortical microglia with Apo-SAA or LPS upregulates, in a time-dependent ...
Figure S3. P2X7Rs do not regulate microglial intracellular pH, IL-1β/IL-18 release, blebbing, or pho...
Figure S2. Microglia P2X7Rs are dynamically regulated by bacterial phagocytosis. (a) The current-vol...
Supplementary figures for cytokines in conditioned medium from microglia show significant upregulati...
Figure S4. P2X7R dye uptake is independent of cell lysis and selective for cations below a certain t...
Figure S2. Simultaneous stimulation of primary microglia with LPS plus IL4. * p < 0.05, ** p < 0.01,...
Cultured human microglia do not bleb in response to ATP. Microglia cultured on chamber slides were e...
PKD family inhibition abrogates LPA-mediated downstream signaling. (A) BV-2 microglia cells were cul...
Figure S1. Survival of primary microglia in the presence of inflammatory mediators. * p < 0.05, ** p...
LPAR5 controls LPA-mediated downstream signaling. (A) BV-2 microglia cells were cultured in 6-well p...
Figure S2. Immunoblotting data of main Fig. 2c, d and f were quantified using Image J software and s...
Figure S1. Immunoblotting data of main Fig. 1a-c were quantified using Image J software. Data were t...
Effects of the AM630 on PGE2 release in LPS-stimulated primary microglial cells. Microglia were pre-...
Figure S1. Purity of microglia cells in culture assessed by immunofluorescence detection of Iba-1- (...
Figure S2. Efficient and selective inactivation of MFP2 in microglia in Cx3cr1-Mfp2−/− mice. (A) Mic...
Figure S1. Treatment of rat cortical microglia with Apo-SAA or LPS upregulates, in a time-dependent ...