Additional file 1: of The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo

Figure S1. Survival of primary microglia in the presence of inflammatory mediators. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to different experimental group as marked by horizontal bar. A) Release of lactate dehydrogenase (LDH) was measured photometrically (LDH assay) as a surrogate for cell death after treatment of microglia with LPS (10 ng/ml), IL4 (50 ng/ml) or both (LPS plus IL4). Lysed cells served as control (n = 7, H(4) = 35.818, p < 0.001). B) Ratio of viable versus dead (propium iodide-positive) microglia subjected to LPS (10 ng/ml), IL4 (50 ng/ml) or both (LPS plus IL4) as assessed by Live/dead assay (n = 4, H(3) = 9207, p < 0.05). (TIF 618 kb