IgA antibody response in BALB/c mice against all five peptides and their mixture inoculated by intranasal route.

<p>Antibody titers in sera of mice primed intranasally with peptide + CFA and boosted twice with peptide + IFA are shown. The X-axis depicts all five peptides, their mixture inoculated with adjuvant and respective control (PBS+ adjuvant) for each peptide. OD values of peptides vs. controls are depicted on Y- axis. Absorbance was measured at 450nm. Data collected from 6 mice per group (2 separate experiments) are expressed as geometric mean concentration (± 2SD) at serum dilution of 1:160. Statistical significance was determined by Mann-Whitney U test and the p-value of < 0.05 was taken as significant.