TNF-α levels in BALB/c mice against all peptides and their mixture inoculated by subcutaneous route.

<p>On the X-axis are shown all five peptides with the adjuvant, their mixture and controls (PBS + Adjuvant) inoculated subcutaneously. Mice were primed with peptide + CFA and two booster doses at two weeks interval each were given with peptide + IFA. TNF-α levels in pg/ml in the undiluted sera are shown on Y-axis. Data collected from 6 mice per group (2 separate experiments) are expressed as geometric mean concentration (± 2SD). Statistical significance was determined by Mann-Whitney U test and the p-value of < 0.05 was taken as significant.