Expression of M2 and NP proteins in A549 cell line transduced with Ad5-tet-M2NP.

<p>(<b>A</b>). Cells were labeled for the M2-protein using M2-specific monoclonal antibodies and Dylight 488 (<b>B</b>). Cells were labeled for the NP-protein using NP-specific monoclonal antibodies and Dylight 488. Vertical lines: left—negative control–cells transduced with Ad5-null (“empty” vehicle); middle—cells transduced with Ad5-tet-M2NP; right—positive control–cells were infected with 869 influenza virus A/USSR/90/77 (H1N1).