DDX39B and DDX39A interact with NP and NS1 protein.

(A, B, C, D and E) HEK293T cells were co-transfected with HA-DDX39B, HA-DDX39A or HA-DDX39B-ck and FlAG-PB2 (A), FlAG-PA (B), FlAG-PB1 (C), FlAG-NP (D) of influenza virus A/Anhui/1/2013 (H7N9) or FlAG-NP of influenza virus A/WSN/1933 (H1N1) (E), at 48h after transfection, the cells were lysed, followed by co-IP with anti-HA mouse Mab and western blotting using anti-FlAG mouse Mab and anti-HA mouse MAb. (F) HEK293T cells were transfected with HA-DDX39B or HA-DDX39A, at 24h after transfection, the cells were infected with recombinant influenza virus WSN-H7 627E at MOI = 1. At 24h after infection, the cells were lysed, followed by co-IP with anti-HA mouse Mab and western blotting using anti-NP mouse Mab and anti-HA mouse MAb. (G) DDX39B-mCherry or DDX39A-mCherry together with NP were transfected into HEK293T cells, 24 hours after transfection, the cells were fixed and examined via fluorescence microscopy. (H) HEK293T cells were co-transfected with HA-DDX39B, HA-DDX39A or HA-DDX39B-ck and FLAG-NS1 of influenza virus A/WSN/1933 (H1N1), at 48h after transfection, the cells were lysed, followed by co-IP with anti-HA mouse Mab and western blotting using anti-FlAG mouse Mab and anti-HA mouse MAb.