THE EFFECT OF EXTRACT ROSELLA (HIBISCUS SABDARIFA L) CALYX ON INCREASING CATALASE ACTIVITY AGAINST 7,12 DIMETHYLBENZ-(A)ANTRASENA (DMBA) INDUCED IN RATS

Background :Free radical is included into a reactive oxygen species (ROS) and reactive nitrogen species (RNS) in the body which can cause degenerative diseases such as neurodegenerative, aging, and cancer e radicals.The excessive concentration of ROS causes oxidative stress as indicated by the activity of catalytic enzyme. Roselle (Hibiscus Sabdariffa L.) has been activity as free radical inhibitors. This study aims to determine the antioxidant activity of ethanol extract of roselle calyx to see cattalase enzyme activity in the treatment of 7 days. Objective :This experimental research was arranged as a post-test controlled group design. Twenty five Male Sprague Dawley rats age of 4 weeks divided into 5 groups: Group are treatment of 7 days, group I are baseline, group II DMBA control (Single dose 75 mg/kg BW), group III, IV and V are roselle extract treatment groups with variations dose group of 10, 50 and 100 mg/kg BW. Methods :Roselle was extracted by maceration 60 % ethanol and followed by evaporator to get concentrated extract of 10, 50 and 100 mg/kg BW. Twenty five Male Sprague Dawley rats age of 4 weeks divided into 5 groups: Group are treatment of 7 days, group I are baseline, group II DMBA control (Single dose 75 mg/kg BW), group III, IV and V are roselle extract treatment groups with variations dose group of 10, 50 and 100 mg/kg BW. Animal was fasted for 16 hours before sampling. after that, the animal were sacrificed and organ were observed using hematocylin and eosin staining, measured cattalase activity. Data were analyzed using Anova, Least Significant Difference, Kruskal-wallis and Mann-whitney. Content of Polyfenol and Flavonoids use gallic acid and quercetin standart. Results : The activity of cattalase was found to increase in catalase enzyme activity of roselle extract at a dose of 10, 50, 100 mg/kg BW in the treatment of 7 days in respectively (5.550 ± 0.683), (6.186 ± 0,764), (5.678 ± 0.813) and the group of DMBA (4.067 ± 0.770). The effect of roselle extract in DMBA control group shows increase in catalase activity of roselle extract dose of 50 mg / kg (6.186 ± 0.764) compared to administration of DMBA (4.067 ± 0.770) (p < 0.05) on day 7 of treatment. Prolonge roselle extract treatment on day 7 to show significant differences in catalase enzyme activity. Conclusion : The rosella flowers ethanol extract has an antioxidant activity showed by the increased catalase activity. Key Word : Hibiscus sabdarifa L, Catalase, DMBA, Antioksida