THE EFFECT OF ROSELLE ( Hibiscus Sabdariffa L.) CALYX ETHANOLIC EXTRACT ON GST ACTIVITY AND DECREASING OF ALT AND AST LEVEL ON 7,12 DIMETHYL BENZ(A) ANTRASEN (DMBA) INDUCED RATS

Background : Oxidants or free radicals caused all abnormalities in this human body and to make liver cells damaged. Various natural products have been observing for drugs or compounds as antioxidants as Roselle ( Hibiscus sabdariffa L ). Objective: The aim of this study was to determine ethanolic extract of roselle protect Method: Roselle was extracted by maceration 60 %ethanol with a ratio of dry calyx and solvents are (1:3) stirer for 24 hours , then is filtered and concentrated by Rotary evaporator at 60°C . Maserate was transferred into a porcelain cup and evaporated on water bath at 60°C . Condensed extract is then stored in a sealed container and be identified .Forty-five Male Sprague Dawley rats age of 4 weeks divided into 9 groups: group I are baseline, group II and III was DMBA control group at day 7 and 35 (Single dose 75 mg/kgbw) . Group IV , V and VI are roselle extract treatment groups with variations each dose group of 10 mg / kg , 50 mg/kg and 100 mg/kgbw on day 7 induced DMBA 75 mg/kgbw. Group VII , VIII and IX treated with roselle extract in dose variation of each group 10 mg/kgbw, 50 mg/kgbw and 100 mg/kgbw. On day 35 induced DMBA 75 mg/kg BB . After that on day 7 and 35 after DMBA single dose, they were fasted (total 5 test animals each group) for 16 hours, then collect blood from eye (orbital plexus) and observed the SGOT and SGPT activity . After that, the animal were sacrified and hepar organ were observe GST gene expression by RT-PCR method. The data were analyzed using Kruska-wallis, Mann-Whitney, ANOVA, Least Significant Difference (LSD). Content of Polyfenol and Flavonoids use gallic acid and quercetin standart. Outcome Measured : - Result&conclusion: Results showed the increasing of ALT level (32.63±0.72) and AST level (27.09±1.51) at DMBA group compared to baseline (p<0.05). Significant differences occurred ALT and AST activity to DMBA control compared (p<0.05), decreasing of ALT and AST activity in a group of 10, 50, 100mg/kg/day compared to DMBA control group, a significant decrease of AST activity 18.45±0.92 (p