SDS-PAGE analysis of lipase from metagenomic SeqA.

Lane M: protein molecular weight marker; Lane C: crude enzyme extract; Lane F1 and F2: unbounded protein fractions; Lane E1: Eluted protein with 100 mM Imidazole. The partially purified enzyme was indicated by arrow. The total protein concentration of eluted protein was 5.62 ± 0.69 mg. (TIF)