The analysis of the lipase activities of the mutants.

<p>The six residue substitutions of site-directed mutagenesis were G49A, G50A, G51A, S88A, K91A, and S361A. (A) A schematic of the LipL protein sequence with predicted catalytic motif and the mutations. (B) The experiment was performed in the standard lipase activity assays. The values represent the means ± SD of three independent experiments.