The quality of the eluted pure recombinant ChO was analyzed using SDS-PAGE 10%. Lane 1: Protein marker; Lanes 2–9: Fractionated samples eluted from Ni-CAM column by 150 mM imidazole.</p
<p>Lane M is protein molecular weight markers. Lane 1 is the crude extract of the recombinant strain...
<p>(A) Lane 1, molecular weight markers (kDa); lane 2, liver extract; lane 3, diethylaminoethyl (DEA...
Cholesterol oxidase is a bifunctional bacterial flavoenzyme which catalyzes oxidation and isomerizat...
Lineweaver-Burk plot for recombinant cholesterol oxidase in the presence of various concentrations o...
Summary of the purification procedure for the recombinant cholesterol oxidase.</p
Lane M: protein molecular weight marker; Lane F1 and F2: unbounded protein fractions; Lane E1: Elute...
Lane M: protein molecular weight marker; Lane C: crude enzyme extract; Lane F1 and F2: unbounded pro...
<p>A. SDS-PAGE analysis. M: Protein markers; Lane 1: cell extract; Lane 2: unbound proteins in the f...
<p>A) SDS-PAGE. Lane 1: Precision Plus All Blue molecular weight standard (Bio-Rad). Lane 2: Soluble...
<p>Five samples with significant oxidase activity were selected, two from the control plate and thre...
<p>SDS-PAGE analysis of the purified recombinant Xyl3A. Lanes: 1, the purified recombinant Xyl3A aft...
<p>M: protein standard marker; Lane 1: the supernatant of the cell-free extract; Lane 2: purified ni...
<p>A represented the SDS-PAGE of whole-cell protein. Lane 1, protein marker; lane 2, the cell withou...
<p>Lane M, protein molecular weight markers; lane 1, the crude extract of the recombinant <i>E</i>. ...
SDS-PAGE analysis of the purified recombinant proteins used in this study. M = molecular mass marker...
<p>Lane M is protein molecular weight markers. Lane 1 is the crude extract of the recombinant strain...
<p>(A) Lane 1, molecular weight markers (kDa); lane 2, liver extract; lane 3, diethylaminoethyl (DEA...
Cholesterol oxidase is a bifunctional bacterial flavoenzyme which catalyzes oxidation and isomerizat...
Lineweaver-Burk plot for recombinant cholesterol oxidase in the presence of various concentrations o...
Summary of the purification procedure for the recombinant cholesterol oxidase.</p
Lane M: protein molecular weight marker; Lane F1 and F2: unbounded protein fractions; Lane E1: Elute...
Lane M: protein molecular weight marker; Lane C: crude enzyme extract; Lane F1 and F2: unbounded pro...
<p>A. SDS-PAGE analysis. M: Protein markers; Lane 1: cell extract; Lane 2: unbound proteins in the f...
<p>A) SDS-PAGE. Lane 1: Precision Plus All Blue molecular weight standard (Bio-Rad). Lane 2: Soluble...
<p>Five samples with significant oxidase activity were selected, two from the control plate and thre...
<p>SDS-PAGE analysis of the purified recombinant Xyl3A. Lanes: 1, the purified recombinant Xyl3A aft...
<p>M: protein standard marker; Lane 1: the supernatant of the cell-free extract; Lane 2: purified ni...
<p>A represented the SDS-PAGE of whole-cell protein. Lane 1, protein marker; lane 2, the cell withou...
<p>Lane M, protein molecular weight markers; lane 1, the crude extract of the recombinant <i>E</i>. ...
SDS-PAGE analysis of the purified recombinant proteins used in this study. M = molecular mass marker...
<p>Lane M is protein molecular weight markers. Lane 1 is the crude extract of the recombinant strain...
<p>(A) Lane 1, molecular weight markers (kDa); lane 2, liver extract; lane 3, diethylaminoethyl (DEA...
Cholesterol oxidase is a bifunctional bacterial flavoenzyme which catalyzes oxidation and isomerizat...
DOI
Add to ORKG