Fig 5 -

Pathway analysis encompassing the top 150 meta-DEGs: (a) Representative network model of interactions across up-regulated and down-regulated genes unravel their role in metabolic and biosynthetic processes respectively. Node colour reflects up-regulation (red ellipses) or down-regulation (green triangles). Interactions are represented by edges, with thicker edges indicating a more significant interaction. The degree distributions of nodes shown are derived on the undirected network of protein-protein interactions. (b &c) The two figures represent data distribution amongst degree versus betweenness-centrality (left) and clustering coefficient versus betweenness-centrality (right). The red line reflects an altered power rule indicating that the network under investigation is scale-free. Protein-protein interactions (PPI) among the 26 DEGs genes: (d) 26 of 150 hub DEGs (18 up-regulated genes (red ellipses), 8 down-regulated genes (red triangles) were premeditated for protein-protein interaction network complex. (e) Venn scheme depicting the intersection of four parameters intended for categorizing the hub genes. The four parameters are represented by assorted coloured regions. The overlapping regions represent the most frequently composed DEGs. The figures in each segment of the Venn scheme denotes the number of up and down-regulated genes respectively. f. Enriched pathway and hub genes disclosed with the aid of ClueGO plugin in Cytoscape: ClueGO plugin offers a comprehensive enrichment analysis for DEGs, including Gene Ontology biological process and KEGG pathways. With a kappa score of 0.4, the network’s connectivity is represented by functional nodes and edges shared by the hub genes. Only significant (i.e. p-value ≤ 0.05) pathways are emphasized in the enrichment. The node size is indicated by the p-value and represent discrete biological process. The colour code of a node displays the functional class in which it is active. Nodes of the same colour belong to the same functional group. Colour that are assorted fit into a diversity of categories. The numerous molecular pathways involved in the enrichment analysis of the known hub genes are represented by unique colour. The bold fonts indicate most significant functional pathways that determine the terms of each group’s signalling pathways. Hub genes linked in each group are highlighted in red font.