The <i>crm1-T539C</i> mutation does not substantially impair meiosis or checkpoint function in the absence of LMB.

(A) Sporulation efficiency was examined after 3 days on sporulation plates. Error bars, SD; n = 3. At least 300 cells were counted for each strain. Strains are: DP421 (wild type), DP1717 (crm1-T539C GFP-PCH2), DP422 (zip1Δ), and DP1721 (zip1Δ crm1-T539C GFP-PCH2). (B) Time course analysis of meiotic nuclear divisions. The percentage of cells containing two or more nuclei is represented. Ethanol (Mock) or Leptomycin B (LMB) were added 15 h after meiotic induction (arrow). Error bars: SD; n = 3. At least 300 cells were scored for each strain at every time point. Strains are: DP1620 (ZIP1 CRM1 GFP-PCH2), DP1717 (ZIP1 crm1-T539C GFP-PCH2), DP1621 (zip1Δ CRM1 GFP-PCH2) and DP1721 (zip1Δ crm1-T539C GFP-PCH2). (TIF)