Cac1 deletion does not affect crossover levels nor spore viability.

<p>(A) Meiotic progression was followed by DAPI staining in WT (VBD1311), <i>cac1Δ</i> (VBD1341) and <i>cac2Δ</i> (VBD1262) strains. Past Meiosis I: nuclei having passed the first meiotic division. (B) Spore viability in WT (VBD1311; 155 tetrads), <i>cac1Δ</i> (VBD1341; 175 tetrads) and <i>cac2Δ</i> (VBD1262; 213 tetrads) strains. (C) Cac1-3HA association with <i>HIS4LEU2</i>, <i>GAT1</i> (DSB1) and <i>BUD23</i> (DSB2) Spo11 sites relative to negative control site. ChIP was performed in a VBD1379 meiotic time course. (D) DSB formation and CO frequency at <i>HIS4LEU2</i> in WT (VBD1311) and <i>cac1Δ</i> (VBD1341) monitored by Southern blot. The graph shows DSB and CO quantification from the same time-courses. (E) CO frequency at the <i>EST3-FAA3</i> locus in <i>ZIP3</i> (VBD1229), <i>zip3-4AQ </i>(VBD1113), <i>cac2Δ ZIP3</i> (VBD1282) and <i>cac2Δ zip3-4AQ</i> (VBD1283) monitored by Southern blot. The positions of parental bands (P1 and P2) and of the recombinant crossover products (CO1 and CO2) are indicated. The graph shows mean CO2 frequency between the 12h and 13h time-points ± standard deviation.