Defective LinE maturation in double <i>cig1 crs1</i> deletion mutants.

(A) Quantification of LinE formation by visualization of Rec25-GFP maturation status during synchronous diploid pat1-114 rec25-GFP meiosis of control (CMC78) and double cig1 crs1 deletion mutants (CMC1207). Graphs represent the percentage of mono-nucleated cells with Rec25-GFP signal (any category), diffuse nuclear signal (earliest transient category visible at S-phase), diffuse nuclear+foci signal (later transient category visible at prophase), and mature signal (clear foci; latest category visible at late prophase). An example of each category is shown in the inset picture in the corresponding graph. (B) Photographs of cells in prophase (Methanol/Acetone fixed) at 3 hr after meiotic induction when maximal maturation of LinEs is observed in the control. DNA (DAPI-staining; left panels), Rec25-GFP signal (middle panels), and both (merge; right panels). Circles indicate cells with Rec25-GFP diffuse nuclear+foci signal and arrows cells with mature signal. At least 45 cells were analyzed at each time point from 1.5 hr after meiotic induction (when signal first appeared), increasing the number of cells to 49–73 during prophase.