Fig 2 -

Phenotype of MOs as determined by βIII Tubulin (TUJ1) and Caspase3 (CAS) immunofluorescence and immunohistochemistry. (A) Immunofluorescence images TUJ1 (magenta) and CAS (yellow) staining in MOs of each line at 5x and 20x magnification, nuclei are stained with DAPI (cyan). Scale bars: 125 μm (5x), 25 μm (20x). Regions of magnification are indicated with white dashed boxes. (B) Total TUJ1-positive area for every line at every time point, data is presented as a bar plot of the mean ± s.d. of the percentage of the total tissue area that was stained positive for TUJ1 for each line. (C) Immunohistochemistry of CAS staining in each organoid line at 4x and 40x magnification. Scale bars: 250 μm (4x), 25 μm (40x). Regions of magnification are indicated with solid red boxes. (D) Total CAS-positive area with data presented as a bar plot of the mean ± s.d. of the percentage of the total tissue area that was stained positive for CAS for each line.