Immunofluorescence analysis of γH2AX foci in Pol β proficient and deficient cells after MMS treatment

<p><b>Copyright information:</b></p><p>Taken from "The accumulation of MMS-induced single strand breaks in G phase is recombinogenic in DNA polymerase β defective mammalian cells"</p><p>Nucleic Acids Research 2005;33(1):280-288.</p><p>Published online 12 Jan 2005</p><p>PMCID:PMC546155.</p><p>© 2005, the authors © </p> Wild-type and Pol β cells were treated with MMS and γH2AX foci analysis was performed. The spatial distribution of γH2AX foci is shown in representative nuclei of wild-type () and Pol β () cells under high magnification. The histogram () reports the percentage of γH2AX foci after cell treatment with 0.5 mM MMS for 15 min and different repair times as indicated. The histogram () reports the percentage of γH2AX foci after cell treatment with increasing MMS doses as indicated. Cells with at least 10 foci were counted as γH2AX positive cells