Time-course of Thr dephosphorylation of SBiP1 after the light stimulus.

A. Upper lanes: western blot with anti-pThr antibodies (lanes SBiP1-p) of total protein extracts from CassKB8 cells adapted to darkness for 12 h (Dark) or after light stimulation for 0.5, 1, 5, 15, or 30 min (lanes 0.5–30 min); lower lanes: western blot with anti-SBiP1 antibodies (lanes SBiP1) of equivalently loaded and treated total protein extracts from CassKB8 cells. B. Densitometric analysis of the SBiP1 Thr phosphorylation level quantitated and normalized to the total SBiP1 levels from three biological replicates of each equivalent treatment.