SBiP1 Thr dephosphorylation levels after stimulation with various light spectra.

A. Upper lanes: western blot with anti-pThr antibodies (lanes SBiP1-p) of total protein extracts from CassKB8 cells adapted to darkness for 12 h (Dark) or after light stimulation with ~ 100 μmole photon m-2 s-1 of blue, yellow, red, or regular white (lanes B, Y, R, W, respectively) light; lower lanes: western blot with anti-SBiP1 antibodies (lanes SBiP1) of equivalently loaded and treated total protein extracts from CassKB8 cells. B. Densitometric analysis of the SBiP1 Thr phosphorylation level quantitated and normalized to the total actin levels from three biological replicates of each equivalent treatment. C. Graph showing the wavelength ranges of the different lights used for stimulation of the dark-adapted CassKB8 cells, each colored according to their visible spectra, except for the white which is shown in grey.