Phosphoprotein activation upon IL-6 stimulation in PHH.

<p><b>(A) Heat map showing mean changes (IL-6 vs. control) in levels of 32 phosphoproteins at two time points from three independent PHH donors.</b> Phosphoproteins were detected by RPA with phosphospecific antibodies directed at the indicated phosphorylation sites. Red color represents positive and blue color negative log2FC (color code provided). Asterisks indicate statistical significance (P<0.05). (B-E, left panel) Bar charts showing means ± SD (n = 3) of relative fluorescent intensities (RFIs) of selected phosphoproteins at different time points after IL-6 stimulation. RFIs were obtained from the RPA and background-normalized. Error bars represent standard deviations calculated from replicates of three donors (not available in E) and asterisks indicate statistical significance (P<0.05). (B-E, right panel) Western blots of total cell lysate (20 µg each) of selected phosphoproteins from control (Ctrl.) and IL-6 treated cells. β-Actin staining served as loading control. Detection was performed with an Odyssey infrared imaging system.