Phosphorylation status of PAR proteins upon incubation of trypomastigotes to laminin, fibronectin or BSA.

<p>(<b>A</b>) Representative immunoblotting of phosphorylated (pS, pT, pY) and PAR proteins immunoprecipitated during the incubation time. (<b>B</b>) Quantitation of 3 independent experiments as described in (<b>A</b>); asterisk represents a comparison between the 5 min and 120 min points by the Student's t-test with p<0.05, indicating a progressive PAR dephosphorylation. (<b>C</b>) Representative immunoblotting of phosphorylated (pS, pT, pY, top) and total (bottom) soluble (S) and insoluble (INS) immunoprecipitated PAR proteins from trypomastigotes incubated for 2 h with BSA, laminin (L) or fibronectin (F). (<b>D</b>) Calculation of the phosphorylation ratio relative to BSA from the experiments in (<b>C</b>); ratio: relative intensity of phosphorylated-PAR treatment/control (BSA); on the left, 80 corresponds to the molecular mass standard in kDa.