Phosphatase activity is needed for <i>C</i>. <i>parvum</i> infectivity.

<p>A) phospho-Tyrosine blot analysis using whole cell lysates from <i>C</i>. <i>parvum</i> infected FHs 74 Int cells at 2h and 4 h post infection. Tyrosine-phosphorylated proteins decrease at higher molecular weights upon infection. B) Treatment of FHs 74 Int cells with the phosphatase inhibitor, sodium orthovanadate significantly decreases <i>C</i>. <i>parvum</i> infectivity. The number of infections per field of view (at 200X magnification) are represented on the y-axis. Values are means ±SD for three independent experiments. Statistical significance criteria set at: *p<0.05, **p<0.02.