Caspase-3 accumulates in LF82 infected RAW 264.7 cells after proteasome inhibition.

<p>(A) Cell death of RAW 264.7 cells at 6 hours post infection (hpi). The percentage of dead cells were calculated using a LIVE/DEAD stain and expressed as a percentage of the total number of cells present per well of a 24 well plate. (B) Accumulation of caspase-3 activity in RAW 264.7 cells after proteasome inhibition from 0–6 hpi. Caspase-3 activity in RAW 264.7 cells was measured at 6 hpi and expressed as activity, measured in fluorescence focus units (FFU) per mg of protein. Measurements were carried out in the presence or absence of 10 µM MG132. (C) Accumulation of caspase-3 activity in RAW 264.7 cells after proteasome inhibition from 24–30 hpi. Caspase-3 activity in RAW 264.7 cells was measured at 30 hpi and expressed as activity, measured in fluorescence focus units (FFU), per mg of protein. Measurements were carried out in the presence or absence of 10 µM MG132. All experiments were repeated at least three times in triplicate and data from representative experiments are shown. Data was analyzed by an unpaired Student’s <i>t</i>-test. Statistically significant relationships are denoted. <i>P</i> values **<0.05, ***<0.005.