Analysis of activated caspases and anti-apoptotic Bcl-2.

<p>A) A representative Western blot of procaspase and cleaved caspase-8 after 12 hours of treatments. B) A representative Western blot of caspase-7 and procaspase-9 after 12 and 24 hours of treatments. C) A representative Western blot of procaspase and cleaved caspase-3 after 12 hours of treatments. Caspase 3 activity was measured by detection of fluorescence of the caspase substrate after 12, 24 and 36 hours of treatments. Data are expressed as relative AFC (7-amino-4-trifluoromethylcpumarin) fluorescence normalized for total protein content in each sample. D) Effect of CA on phosphorylated Bcl-2 protein levels at 12 and 24 hours of treatments. Images report one representative experiment out of at least 3 independent experiments.*p<0.05 <i>vs</i> control; **p<0.001 <i>vs</i> control; # p<0.05 CA+HG <i>vs</i> HG.