RhCMV viral kinetics in blood and body fluids of individual CD4+ T lymphocyte depeleted CMV-seropositive macaques.

RhCMV in plasma (indicated in red), saliva (blue), urine (orange), and amniotic fluid (purple) in (A) five CMV-seropositive reinfected animals and (B) three CMV-seropositive control animals. Plasma and amniotic fluid are reported in mean RhCMV DNA copy number/mL of sample fluid; saliva and urine are reported as mean RhCMV DNA copy number/μg of input DNA. In CMV-seropositive controls, the equivalent post-infection time-points on the x-axis are aligned concurrently with the CMV-seropositive Reinfection group. The black vertical lines indicate time of anti-CD4 antibody (CD4R1) and RhCMV inoculation. Animals JP01, KK24, and KB91 were inoculated with RhCMV UCD52 and FL-RhCMVΔRh13.1/SIVgag; animals 274–05 and 292–09 were inoculated with RhCMV 180.92; animals 234–07, 309–09, and 222–02 remained without a reinfection. The horizontal stippled line indicates the baseline mean RhCMV DNA copy number/μg of input DNA in either saliva (blue) or urine (orange).