FIGURE 2 from Pancreatic Ductal Adenocarcinoma Cells Regulate NLRP3 Activation to Generate a Tolerogenic Microenvironment
- Jesus Amo-Aparicio (17013541)
- Adrian Dominguez (17013544)
- Shaikh M. Atif (17013547)
- Alberto Dinarello (17013550)
- Tania Azam (17013553)
- Kibrom M. Alula (17013556)
- Miles Piper (15035907)
- Christopher H. Lieu (15018279)
- Robert W. Lentz (17013559)
- Alexis D. Leal (17013562)
- Stacey M. Bagby (17013565)
- Wells A. Messersmith (17013568)
- Sana D. Karam (17013571)
- Charles A. Dinarello (17013574)
- Todd M. Pitts (17013577)
- Carlo Marchetti (17013580)
Publication date
September 2023
DOIAbstract
PDAC paracrine activity mediates NLRP3 activation. A, IL1β and IL18 secretion from freshly isolated PBMCs from healthy donors (n = 6) following stimulation with PDAC-conditioned media obtained from AsPC-1, MiaPaCa-2, and Panc-1 cells. B, IL1β and IL18 secretion from parenteral (WT) and NLRP3 deficient (NLRP3−/−) THP-1 cells following stimulation with PDAC-conditioned media obtained from Panc-1 cells (n = 3). C, IL1β and IL18 secretion from THP-1 cells following stimulation with PDAC-conditioned media obtained from Panc-1 cells in presence of the NLRP3 inhibitors OLT1177 (OLT) and MCC950 (MCC; n = 3). Cytokine levels were measured by specific ELISA kits. Data expressed as mean ± SEM; ****, P P P < 0.05.</p
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