Ct-αSyn complex vaccine downregulates synaptic markers.

Vaccinated mice (n = 7; Vac + αSyn) and non-vaccinated mice (n = 4; PBS + αSyn) were administered with the viral vector AAV1-αSyn in the right substantia nigra for overexpressing human αSyn. (A, B) Twenty weeks later, striatum (STM) tissues of the right hemisphere brain were examined by western blot analysis to probe synaptophysin, postsynaptic density protein-95 (PSD95), and β-actin. The molecular weight (kDa) and migration location of protein markers are indicated. (C, D) The protein levels of synaptophysin and PSD95 were measured and normalized to β-actin by densitometric analysis. Data are expressed as mean values ± SEM, and significant differences between groups are indicated (*p t-test).