Fig. 5 in Cytochrome P450 catalyses the 29-carboxyl group formation of celastrol

Fig. 5. The role of TwCYP712K1 in celastrol biosynthesis. (a) Network of differentially expressed CYP450s and TwOSC involved in the biosynthesis of celastrol. Transcripts are represented by circles and metabolites by squares and edges are drawn when the linear correlation coefficient is> 0.7 in that tissues. The size of each circle represents the amount of expression of the gene. The pie chart shows the ratio of gene accumulation expression in different tissues. Colour key: the root was represented by purple, the stem periderm by orange, leaves by green, the stem vascular bundle by blue and flowers by red. (b) Relative expression of TwCYP712K1 in RNAi suspension cells and control suspension cells. (c) Relative expression of TwCYP712K1 in the overexpression suspension cells and control suspension cells. (d) Celastrol content in the RNAi suspension cells, overexpression suspension cells and their control suspension cells. Student's t-test was used to test for significant differences in gene expression levels and celastrol levels between the control group and experimental group. The data represent the average ± SD of at least three independent lines of suspension cells. CK, control group; OE, overexpression group; and RI, RNA interference group. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)Published as part of Zhou, Jiawei, Hu, Tianyuan, Liu, Yuan, Tu, Lichan, Song, Yadi, Lu, Yun, Zhang, Yifeng, Tong, Yuru, Zhao, Yujun, Su, Ping, Wu, Xiaoyi, Huang, Luqi & Gao, Wei, 2021, Cytochrome P450 catalyses the 29-carboxyl group formation of celastrol, pp. 1-10 in Phytochemistry (112868) 190 on page 6, DOI: 10.1016/j.phytochem.2021.112868, http://zenodo.org/record/825807