Images from the eye of a JUNV infected day 12 animal.

Images a and b are from the same region and images c and d represent blue boxes in images a and b respectively. Image a (4X magnification, H&E) shows a thickened choroid; retina is artificially detached from choroid. In image b (4X magnification, ISH) there is strong viral RNA labeling (red) in choroid extending anterior toward the anterior uvea (black arrow); note also the small section of lacrimal gland with a minimal of labeling (blue arrow); part of the sclera is artifactually separated from the choroid. Image c (20X magnification, H&E) shows the choroid (between thick black arrows) thickened with infiltrating mononuclear inflammatory cells; note the retina is artifactually separated with only thin layer of outer retina remaining attached. Image d (20X magnification, ISH) shows strong viral RNA labeling in the inflamed, thickened choroid; note the sclera has partially, artifactually separated from the choroid).