Images from the lung of a GTOV infected day 12 animal.

Images a and b are from the same region; images c and d represent blue boxes in images a and b respectively. In image a (4X magnification, H&E) there are multifocal areas of minimal alveolar inflammation (blue arrow and blue box) that are often associated with small vessels, and areas of mononuclear infiltrate surrounding larger vessels (black arrow). In image b (4X magnification, ISH) there is strong viral RNA labeling in areas of alveolar inflammation and labeling in mononuclear infiltrates surrounding vessels. In image c (20X magnification, H&E) there is minimal mononuclear alveolar inflammation, at the center of which is a small vessel surrounded by a mononuclear infiltrate (perivascular). Image d (20X magnification, ISH) is the corresponding ISH image and shows strong viral RNA labeling in the area of alveolar inflammation and surrounding the small vessel at center.