Expression analysis of antiviral genes using quantitative RT-PCR.

qRT-PCR analysis of the antiviral genes. Twenty-four hours after introducing the LAB genomes using lipofection, total RNA was extracted from HT-29 cells and subjected to qRT-PCR. Mock (non-treated), Poly(I:C) (Poly(I:C) treated), and LAB (LAB genome introduced) were analyzed. Gapdh was used as an internal control, and the results are shown relative to the expression of the mock control. Values are presented as mean ± S.E. (n = 4). Asterisks indicate a statistically significant difference compared with the mock control analyzed using Student’s t-test (*P P MX1. (B) Expression of OAS1.