Effect of fiber width on adhesion stability.

A. Average percentage of ligated integrins varying the width of the actin fiber and using Pbundling = 1. Errorbars indicate standard deviation from the mean. B. Number of binding events per second varying the width of the actin fiber and using Pbundling = 1. C. Distribution of total time in the ligated state, calculated as sum of the ligand-bound lifetimes of for each integrin over the course of 500 s of simulations, using Pbundling = 1 and increasing the width of the fiber. D. Average angle of integrin adhesion, relative to the direction of the actin fiber, using Pbundling = 1 and varying the width of the fiber. The angle is calculated from the direction of the first principal component of the 2D positions of ligated integrins. Errorbars indicate standard deviation from the mean. All data are computed in the absence of actomyosin contractility and are evaluated between 100–500 s of simulations, from 3 independent runs using k = 0.6 pN/nm and considering the total number of ligated integrins every 1 s of simulations. (TIFF)