Actomyosin force combined with substrate stiffness result in load-and-fail of adhesions.

A. A heatmap of the average percentage of ligand bound integrins varying substrate stiffness, k, between 0.1–0.7 pN/nm, and actomyosin force, Fmyo, between 0–40 pN. The reported values are averages between 100–300 s of simulations. B. A heatmap of the total ligated time of integrins during 300 s of simulations, varying k between 0.1–0.7 pN/nm and Fmyo between 0–40 pN. C. The average percentage of integrins undergoing unbinding every second of simulations, varying Fmyo between 0–40 pN, using k = 0.1, 0.4, and 0.7 pN/nm. Data are extracted between 100–300 s of simulations.