<i>flp-1</i> significantly suppresses the exploration defect of <i>grk-2</i>.

(A) grk-2(gk268) mutant animals have an exploration defect and flp-1(ok2811) significantly suppresses this defect. Shown is the number of squares (out of 50) single animals covered in ~20 h. (****, Pflp-1 and flp-18 act in the same pathway to regulate exploration. flp-1(ok2811) and flp-18(db99) partially suppress the exploration defect of grk-2(gk268) mutant and the grk-2(gk268); flp-1(ok2811); flp-18(db99) triple mutant behaves like the grk-2(gk268); flp-1(ok2811) double mutant. (****, P0.05. One way ANOVA followed by a Bonferroni test. Error bars = SEM; n = 20–28). (C) Expression of flp-1 under its own promoter (flp-1p::flp-1, transgene yakEx231) or under the twk-47 promoter (expression in AVK neurons; twk-47p::flp-1, transgene yakEx216), reverses the flp-1 suppression of the exploration defect of grk-2(gk268) mutant. (****, Pnpr-1(ky13) suppresses the dispersal defect of grk-2(gk268) mutant and the grk-2(gk268); flp-1(ok2811); npr-1(ky13) triple mutant behaves like the grk-2(gk268); npr-1(ky13) double mutant. Shown is the distance (cm) animals traveled from the center of the plate in the presence of food in 1 h. (ns, P>0.05. One way ANOVA followed by a Bonferroni test. Error bars = SEM; n = 51–67). (E) npr-1(ky13) suppresses the exploration defect of grk-2(gk268) mutant and the grk-2(gk268); flp-1(ok2811); npr-1(ky13) triple mutant behaves like the grk-2(gk268); npr-1(ky13) double mutant. Shown is the number of squares (out of 50) single animals covered in ~20 h (ns, P>0.05. One way ANOVA followed by a Bonferroni test. Error bars = SEM; n = 20–23). (F) frpr-7(gk463846) partially but significantly suppresses the exploration defect of grk-2(gk268) mutant. npr-6(tm1497) has no effect on grk-2(gk268) exploration. (***, P0.05. One way ANOVA followed by a Bonferroni test. Error bars = SEM; n = 23–39).