<i>egl-4</i> suppresses the exploration defect, movement quiescence, and reduced activity of <i>grk-2</i> mutant animals.

(A), (B) The egl-4(n479) mutation partially suppresses the exploration defect of grk-2(gk268) mutant as assayed on 6 cm (A) and 10 cm (B) plates. Shown is the number of squares (out of 50, (A) or 150, (B)) single animals covered in ~20 h. Please note that egl-4 mutant animals explore more than wild type animals as assayed on 10 cm plates. (****, P0.05. One way ANOVA followed by a Bonferroni test. Error bars = SEM; n = 22–26). (C) Expression of egl-4 cDNA in ciliated sensory neurons under the osm-6 promoter reverses the egl-4 suppression of the exploration defect of grk-2 mutant (osm-6p::egl-4, transgene yakEx221). Shown is the number of squares single animals covered in ~20 h (****, P0.05. Student’s t-test. Error bars = SEM; n = 31–33). (D) grk-2(gk268) mutant animals show enhanced movement quiescence, and the egl-4(n479) mutation suppresses this phenotype. The graph shows the average minutes animals spent in quiescence per hour in the WorMotel. Quiescence is the time when zero worm pixels moved between two frames separated by 10 sec (See Methods). One WT animal was considered an outlier and was not included in the graph since its values were more than 3 standard deviations from the mean. (E) The graph shows the total minutes individual animals spent in quiescence in 8 h in the WorMotel. (****, P0.05. One way ANOVA. Error bars = SEM; n = 11–12). (F) grk-2(gk268) mutant animals show reduced activity, and the egl-4(n479) mutation partially suppresses this phenotype. The graph shows the activity in pixels of individual animals in 8 h in the WorMotel. Activity is the number of pixels moved between frames separated by 10 sec. (****, P<0.0001. One way ANOVA. Error bars = SEM; n = 11–12).