FLP-20 is involved in TRN neuropeptide signaling modulating locomotion.

<p>(A) <i>flp-20</i> mutants have an increased off-food reversing rate that is smaller than the <i>mec-4</i> increase. <i>flp-20; mec-4</i> double mutants exhibit a similar increase in off-food reversing rate as <i>mec-4</i> single mutants. (B) An additional <i>flp-20</i> allele displays a similar increase in reversing frequency. (C) TRN-specific expression of FLP-20 cDNA restores <i>flp-20</i> reversing rate off-food. (D) TRN-specific expression of FLP-20 cDNA reduces <i>flp-20</i> reversing rate off-food, but not on-food (2-way ANOVA interaction <i>p</i> = 0.0005). (E) Eliminating functional FLP-20 sequence exclusively in the TRNs is sufficient for increasing reversing rate. (F) Overexpression of FLP-20 in the TRNs of <i>mec-4(253)</i> mutants decreases their reversing rate. (G) TRN photo-stimulation has a weakened suppressive effect on the reversing rate of Mec-deficient worms lacking functional FLP-20. (H) Pflp-20::GFP fluorescence intensity, indicating FLP-20 transcription, in <i>mec-4(u253)</i> mutants and following disruption of exocytosis in the TRNs is reduced in ALM, does not substantially vary in PVC, and is also reduced in the ASE neurons (2-way ANOVA interaction <i>p</i> < 0.0001). Sample size indicated in each panel; Error bars represent SEMs; *<i>p</i> < 0.05, **<i>p</i> < 0.01, ***<i>p</i> < 0.001, ****<i>p</i> < 0.0001 <i>t</i> test with Bonferroni corrections for multiple comparisons where relevant.