Some different procedures for the evaluation of the non-linear behavior of masonry arch bridges are here proposed. In particular, the Venice trans lagoon masonry arch bridge is numerically analyzed by means of several 3D FE numerical strategies. The three dimensional behavior of the structure when subjected to train loads and pile foundation settlement is investigated. Focus is also posed to the stabilizing role played by the backfill. Both a non-commercial code and a standard commercial FE software are utilized. The non-commercial code properly takes into account for the orthotropic behavior of the barrel vaults and the stone arches, and allows performing non-linear static and limit analyses on complex 3D structures. Within the noncommerci...