Distribution of DNA modification sites along the genome of <i>M</i>. <i>gallisepticum</i> S6.

A–Annotated features: RM systems are in orange, vlhA clusters in green, the CRISPR system in grey, the virulence cluster in blue, and mobile elements in black. B and С –Whole-genome representation of the DNA modification motif ANCNNNNCCT for plus and minus strand, respectively. The lines indicate positions of sites, and the colors from red to blue represent percentage of methylation in each site. Percentage of methylation was calculated using the build-in SMRT protocol, as the fraction of reads aligning to the position that has a modified base. D–Density of methylation sites. Maximum density of methylation is highlighted in orange, while minimum is highlighted in green. E–GC content. Maximum GC content is highlighted in red, while minimum is highlighted in blue. F–Hypo-methylated sites are in green, hemi-methylated sites in orange and blue (for plus and minus strands, respectively); proteins with differential abundance (as assessed using 2D-DIGE) between WT and ΔS.MgaS6I strains are in grey.