Site-specific analysis of DNA methylation by bisulfite sequencing.

<p>A: <i>EgDEF1</i> gene; B: <i>Koala</i> retrotransposon; C: <i>Rider</i> retrotransposon. The percentage of methylated CG, CHG and CHH sites (where H is A, T or C) within each region investigated is represented as red, blue and green bars respectively. The average methylation rate throughout each sequence is represented as diamonds (because of the scale, these percentages could not be represented on the <i>EgDEF1</i> graph). N, M: inflorescence sampled on a clonal oil palm of either normal or <i>mantled</i> floral phenotype, respectively. The localization of PCR fragments on their respective target sequence is as indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091896#pone-0091896-g001" target="_blank">Figure 1</a>.