Methylation of <i>M</i>. <i>hyorhinis</i> genomic DNA.

<p>A and B, schematic diagrams show the presence of two GATC and 27 CG methylation sites in the 368 bp CG-rich region of the <i>M</i>. <i>hyorhinis</i> genome in free-living (A) and intracellular (B) mycoplasmas. Circles indicate methylated CGs and GATC sites (red and blue, respectively), and unmethylated (empty) sites. Each row corresponds to a methylation pattern in the individual mycoplasma cell. Bottom, a fragment of the sequencing chromatogram of the bisulfate-converted DNA confirms the presence of the methylated GATC and CG sites in the 368 bp CG-rich region of the <i>M</i>. <i>hyorhinis</i> genome. Stars, 5mC. C, Circular histogram shows the distribution of the CG and GATC sites in the <i>M</i>. <i>hyorhinis</i> genome. Histogram bar heights correspond to a number of the individual CG and GATC marks (light red and light blue, respectively) within a 1 kb window. Regions with >10 sites are shown in dark red (CG) and dark blue (GATC). Genes are named and their positions are shown along the chromosome as orange bars. In the genomic DNA, positions of mycoplasma CPGI-like regions (mCPGIs) are indicated by thin red lines on the white background. The histogram was generated using Circos [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0142529#pone.0142529.ref026" target="_blank">26</a>]. D, Dinucleotide analysis of <i>M</i>. <i>hyorhinis</i> genome.