Technical replicates of the sequencing of the virus library.

Using Illumina sequencing, one replicate of the virus library was sequenced in one run and three additional technical replicates were sequenced in a separate run. Thus, the four technical replicates were sequenced in two independent runs. The UpSet plot shows the number of barcodes that intersect amongst the four virus library technical replicates (lower panel), as well as the total counts of those barcodes in blue (upper panel). The libraries were clustered using L3 distance and a 99% reads cutoff was applied. The four replicates show a large degree of overlap in clustered barcodes.