Barcode shuffling during multiplexed Mosaic-seq library preparation.

<p>(A) Summary of the sample conditions. (B) Read distribution of three representative libraries. For each barcode, we only observed one dominant sgRNA sequence, which is always the expected sgRNA. (C) The percentage of reads for the most abundant sgRNA for each barcode are plotted in the boxplot. Whiskers represent the 10th and 90th percentiles, and the dots represent outliers. Virus infection in the left panel were performed by using the same volume of virus stock (MOI varies from 1-2.4); right panel shows an independent experiment with high and low MOI, using the same virus packaged in the left panel.